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Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis
J J Kupiec1, M L Giron, D Vilette
1UER d'Hématologie, LOI CNRS, Hôpital Saint-Louis, Paris, France.
Analytical Biochemistry
|July 1, 1987
Abstract:
A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.