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Updated: Mar 7, 2026

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
Published on: October 13, 2021
Facile fabrication of polyurethane microcapsules carriers for tracing cellular internalization and intracellular
Yuqing Niu1, Florian J Stadler2, Jun Song3
1Nanshan District Key Laboratory for Biopolymers and Safety Evaluation, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Polymer Science and Technology, Shenzhen 518060, PR China; Guangdong Research Center for Interfacial Engineering of Functional Materials, Shenzhen 518060, PR China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, PR China; Shenzhen University Health Science Center, Shenzhen 518060, PR China.
Abstract:
A tailor-made traceable pH-sensitive drug delivery system based on polyurethane (PU) microcapsules was fabricated using a facile double-emulsion method containing 3,3'-dioctadecyloxacarbocyanine perchlorate, doxorubicin (DOX) and sodium bicarbonate (NaHCO3). When PU microcapsules were immersed in acidic media, NaHCO3 could react with the H+ to quickly produce CO2 bubbles to puncture the PU shell, resulting in rapid release of DOX to promptly reach the intracellular drug therapeutic threshold to kill cancer cells in a short period. Confocal laser scanning microscopic analysis showed that these traceable pH-sensitive drug carriers can be easily internalized by BGC 823 and Hela cells, and the loaded DOX can quickly release from PU microcapsules in the endo-/lysosomes to be mainly resided in cell nuclei. This traceable pH-sensitive drug carrier can achieve on-demand controlled release profiles for visualization of cancer therapy. Thus, it is a potential candidate for anticancer drug delivery system in advanced cancer therapy.
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