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Inactivation of herpes simplex virus by protein components of bovine neutrophil granules
A Zerial1, B Skerlavaj, R Gennaro
1Rhône Poulenc Santé, Centre de Recherches de Vitry, Vitry-sur-Seine, France.
Abstract:
From an acid extract of granules of bovine neutrophils we isolated fractions of cationic proteins, exhibiting significant anti-herpesvirus activity at concentrations which were devoid of cytotoxicity and of activity against a picornavirus (rhinovirus). The mechanism of action seems to involve a direct neutralization of the virions. Two antiviral peptides with an approximate MW 7500 were purified to homogeneity by reversed phase high-performance liquid chromatography. Proline and arginine accounted for about 43% and 26-27% of their amino acid residues. One of these peptides (IIIa2 beta) had an MIC of 2 microM.
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