Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: The tumor suppressor protein PML controls apoptosis induced by the HIV-1 envelope.

Cell death and differentiation·2026
Same author

Impaired autophagy increases susceptibility to endotoxin-induced chronic pancreatitis.

Cell death & disease·2020
Same author

The negative impact of antibiotics on outcomes in cancer patients treated with immunotherapy: a new independent prognostic factor?

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

T-cell bispecific antibodies in node-positive breast cancer: novel therapeutic avenue for MHC class I loss variants.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

MDM2-TP53 Crossregulation: An Underestimated Target to Promote Loss of TP53 Function and Cell Survival.

Trends in cancer·2018
Same author

Predictors of responses to immune checkpoint blockade in advanced melanoma.

Nature communications·2017

Related Experiment Video

Updated: Mar 7, 2026

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

18.4K

Automated Analysis of Fluorescence Colocalization: Application to Mitophagy.

A Sauvat1, H Zhou2, M Leduc1

  • 1Metabolomics and Cell Biology Platforms, Gustave Roussy Comprehensive Cancer Institute, Villejuif, France; Equipe 11 labellisée Ligue contre le Cancer, Centre de Recherche des Cordeliers, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Université Pierre et Marie Curie, Paris, France.

Methods in Enzymology
|February 27, 2017
PubMed
Summary

This study presents an image-based method to quantify mitochondrial Parkin translocation, a key step in mitophagy. This technique aids in understanding cellular homeostasis and diseases like Parkinson linked to mitophagy dysfunction.

Keywords:
AutophagyBiosensorHigh contentImage analysisParkin

More Related Videos

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes
11:59

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

Published on: May 19, 2023

3.2K
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

6.4K

Related Experiment Videos

Last Updated: Mar 7, 2026

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

18.4K
Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes
11:59

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

Published on: May 19, 2023

3.2K
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

6.4K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Mitophagy is crucial for cellular and organismal homeostasis by removing damaged mitochondria.
  • Dysfunctional mitophagy, involving proteins like PINK1 and Parkin, is linked to Parkinson disease.
  • Mitochondrial quality control is essential for preventing reactive oxygen species production and maintaining genomic stability.

Purpose of the Study:

  • To detail an image-based approach for quantifying mitochondrial Parkin translocation.
  • To provide a method for studying the initiation of mitophagy.
  • To facilitate research into the mechanisms underlying mitophagy and its role in disease.

Main Methods:

  • Development of an image-based assay.
  • Quantification of Parkin translocation to mitochondria.
  • Utilizing fluorescence microscopy for Parkin localization analysis.

Main Results:

  • Successfully established an image-based method for Parkin translocation measurement.
  • Demonstrated the utility of the assay in studying mitophagy initiation.
  • Provided quantitative data on mitochondrial Parkin recruitment.

Conclusions:

  • The developed image-based approach enables robust quantification of mitochondrial Parkin translocation.
  • This method is valuable for investigating mitophagy mechanisms and Parkinson disease pathogenesis.
  • Accurate measurement of Parkin translocation is key to understanding mitophagy regulation.