Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

6.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
6.0K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.4K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative identification of Contracaecum sp. (Nematoda: Anisakidae) from grey mullet Mugil cephalus and cormorant Phalacrocorax gaimardi in the Southeastern Pacific Ocean off Peru.

Veterinary parasitology, regional studies and reports·2025
Same author

Monitoring spatiotemporal changes in chaperone-mediated autophagy in vivo.

Nature communications·2020
Same author

The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots.

Food microbiology·2019
Same author

Algorithm based on the Thomson problem for determination of equilibrium structures of metal nanoclusters.

The Journal of chemical physics·2017
Same author

Tuberculosis Diagnostic Committees' contribution to the National TB Program in Manila and Quezon Cities.

Public health action·2015
Same author

Developments in clinical trials: a Pharma Matters report.

Drugs of today (Barcelona, Spain : 1998)·2014
Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026
Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026
Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026
Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026
Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026
Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.6K

Methods to Study Chaperone-Mediated Autophagy.

E Arias1

  • 1Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY, United States.

Methods in Enzymology
|February 27, 2017
PubMed
Summary
This summary is machine-generated.

Chaperone-mediated autophagy (CMA) degrades cytosolic proteins to maintain cell health and adapt to stress. This review covers methods to assess CMA activity in animal tissues and cell cultures, crucial for understanding related diseases.

Keywords:
AutophagyChemical modulatorsFluorescent reportersLysosomesOrganelle isolation

More Related Videos

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

32.4K
The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

18.8K

Related Experiment Videos

Last Updated: Mar 7, 2026

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.6K
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

32.4K
The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

18.8K

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Chaperone-mediated autophagy (CMA) is a key pathway for degrading cytosolic proteins within lysosomes.
  • CMA is vital for maintaining proteostasis and cellular adaptation to various stresses.
  • Dysfunctional CMA is implicated in numerous human diseases, including neurodegeneration, cancer, and diabetes.

Purpose of the Study:

  • To review and summarize experimental methods for evaluating CMA activity.
  • To provide insights into assessing CMA in both animal organs and in vitro cell cultures.

Main Methods:

  • The chapter reviews established and emerging techniques for CMA assessment.
  • Methods discussed include those applicable to ex vivo tissue samples and in vitro cellular models.

Main Results:

  • A comprehensive overview of diverse experimental approaches for CMA evaluation is presented.
  • The review highlights the applicability of these methods across different biological systems.

Conclusions:

  • Accurate monitoring of CMA activity is essential for understanding its role in health and disease.
  • The reviewed methods offer valuable tools for researchers investigating CMA pathways and related pathologies.