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Cell-cycle dependence of dolichyl phosphate biosynthesis
1Department of Biochemistry, University of South Florida College of Medicine, Tampa 33612.
Archives of Biochemistry and Biophysics
|November 1, 1987
Summary
Dolichyl phosphate levels rise during the cell cycle in L-1210 cells. Key enzymes involved in dolichyl phosphate metabolism show cell-cycle dependent activity, suggesting their role in regulating its levels.
Area of Science:
- Biochemistry
- Cell Biology
Background:
- Dolichyl phosphate is crucial for protein glycosylation.
- Its biosynthesis regulation is not fully understood.
Purpose of the Study:
- To investigate the cell-cycle dependence of dolichyl phosphate biosynthesis.
- To identify enzymes that may regulate dolichyl phosphate levels.
Main Methods:
- Mouse L-1210 cells were fractionated using centrifugal elutriation.
- Cell-cycle progression and enzyme activities were analyzed.
Main Results:
- Dolichyl phosphate levels increased linearly throughout the cell cycle, peaking in late S phase.
- Activities of cis-prenyltransferase, dolichol kinase, and dolichyl pyrophosphatase varied with the cell cycle.
- Dolichyl monophosphatase activity remained constant across the cell cycle.
Conclusions:
- Enzymes like cis-prenyltransferase and the kinase/phosphatase couple likely regulate dolichyl phosphate levels.
- These findings provide insights into the cell-cycle control of dolichyl phosphate metabolism.