Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

5.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.7K
The Central Dogma01:20

The Central Dogma

34.7K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
34.7K
The Central Dogma01:25

The Central Dogma

143.2K
Overview
143.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.9K
Overview
80.9K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

858
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
858
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

785
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
785

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl-tRNA Synthetase Boosts Genetic Code Expansion in E. coli.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Novel genomically engineered antibiotic-free whole-cell biocatalysts for PET hydrolysis and waste remediation.

Journal of hazardous materials·2026
Same author

Propagation of Photoinduced Electric Field Changes Through Phytochrome and their Impact on Conformational Transitions.

Chemphyschem : a European journal of chemical physics and physical chemistry·2025
Same author

Smart co-delivery of Erlotinib and Camptothecin using silica-coated gold nanorods functionalized with recombinant anti-bone morphogenetic protein receptor type I (BMPR-AI).

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Enabling Fluorescence Lifetime Imaging Multiplexing Using UnaG through Its Modification with Canonical and Noncanonical Amino Acids.

ACS sensors·2025
Same author

Introduction: "Noncanonical Amino Acids".

Chemical reviews·2025

Related Experiment Video

Updated: Mar 7, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.8K

Coupling genetic code expansion and metabolic engineering for synthetic cells.

Jan-Stefan Völler1, Nediljko Budisa1

  • 1Department of Chemistry, Technische Universität Berlin, Müller-Breslau-Straße 10, 10623 Berlin, Germany.

Current Opinion in Biotechnology
|February 27, 2017
PubMed
Summary

This study introduces cost-effective in situ production of noncanonical amino acids (ncAAs) for orthogonal protein translation. By engineering cells with new biosynthetic pathways and orthogonal aminoacyl-tRNA synthetase/tRNA pairs, it enables broader chemical diversity for biotechnology.

More Related Videos

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

66.5K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

14.0K

Related Experiment Videos

Last Updated: Mar 7, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.8K
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

66.5K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

14.0K

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Biochemistry

Background:

  • Orthogonal protein translation using noncanonical amino acids (ncAAs) is a standard bioscience technique.
  • Limited efforts exist for systematic, low-cost in situ production of ncAAs.
  • Expanding chemical diversity in cells is crucial for biotechnological applications.

Purpose of the Study:

  • To engineer cells for cost-effective, in situ production of ncAAs.
  • To couple novel biosynthetic pathways with orthogonal aminoacyl-tRNA synthetase/tRNA pairs (o-pairs).
  • To enable peptide/protein production beyond the canonical set and expand cellular chemistry.

Main Methods:

  • Designing and implementing novel biosynthetic pathways within host cells.
  • Utilizing orthogonal aminoacyl-tRNA synthetase/tRNA pairs (o-pairs) for ncAA incorporation.
  • Integrating genetic code expansion (GCE) with metabolic engineering strategies.

Main Results:

  • Demonstrated a method for engineered in situ production of ncAAs.
  • Established a system coupling biosynthetic pathways with o-pairs for GCE.
  • Laid the groundwork for cost-effective bio-production of novel peptides and proteins.

Conclusions:

  • Coupling GCE with metabolic engineering is essential for industrial biotechnology.
  • Engineered cells with novel pathways and o-pairs offer cost-effective solutions for bio-production.
  • This approach transforms orthogonal translation from academic research to industrial application.