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Updated: Mar 7, 2026
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
Published on: November 13, 2015
A Gold@Polydopamine Core-Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem
Chun Kit K Choi1, Chung Hang J Choi2,3, Liming Bian4,5
1Department of Electronic Engineering (Biomedical Engineering), The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Abstract:
MicroRNAs (miRNAs) represent an emerging class of biomarkers for studying and understanding biological events; the development of viable tools for detecting or monitoring the intracellular expression levels of specific miRNAs is of great interest to life scientists and biomedical engineers. Here, we describe the fabrication of a novel class of core-shell nanoprobes that comprise a gold nanoparticle core and a polydopamine (PDA) shell. Our nanoprobes can be used to specifically track the expression profiles of two miRNA markers of osteogenic differentiation (i.e., osteogenesis), namely, miR-29b and miR-31, in differentiating human mesenchymal stem cells (hMSCs). The newly designed nanoprobes may hold great promise in the noninvasive investigation of the long-term dynamics of cellular events such as stem cell differentiation.
