Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.6K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microneedle array platforms for drug delivery and biomarker sensing: From skin mechanics guided design to scalable manufacture for clinical utility.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Virulence potential of polysaccharide dependent biofilm forming methicillin resistant Staphylococcus epidermidis: A differential proteomics approach.

Microbial pathogenesis·2026
Same author

Engineering non-ribosomal peptide synthesis: tuning the antibiotics engine of the microbial world.

Critical reviews in biotechnology·2026
Same author

Diagnostic Value of IgG and IgM Antibodies in Breastfeeding Mothers Infected With Cytomegalovirus (CMV).

Scientifica·2025
Same author

Synthesis, Biological Evaluation and Molecular Docking Studies of Novel 4-Propylsulfonylpiperazines-Based Thiosemicarbazones as Ecto-5'-Nucleotidase and NTPDase Inhibitors.

Archiv der Pharmazie·2025
Same author

Determination of protein transporter function using Raman spectroscopy.

Microbiology (Reading, England)·2025

Related Experiment Video

Updated: Mar 7, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

14.0K

Iridescence in Meat Caused by Surface Gratings.

Juan Leonardo Martinez-Hurtado1, Muhammad Safwan Akram1, Ali Kemal Yetisen2

  • 1Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK. msa40@cam.ac.uk.

Foods (Basel, Switzerland)
|February 28, 2017
PubMed
Summary

Iridescent colors in pork are caused by light diffraction from muscle surface gratings, not quality indicators. This study quantifies iridescence, offering methods to reduce this misleading optical effect.

Keywords:
diffraction gratingdryingiridescencemeatmuscle tissuequality

More Related Videos

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System
12:08

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System

Published on: July 18, 2015

11.2K
Writing Bragg Gratings in Multicore Fibers
08:48

Writing Bragg Gratings in Multicore Fibers

Published on: April 20, 2016

8.7K

Related Experiment Videos

Last Updated: Mar 7, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

14.0K
Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System
12:08

Fabrication of High Contrast Gratings for the Spectrum Splitting Dispersive Element in a Concentrated Photovoltaic System

Published on: July 18, 2015

11.2K
Writing Bragg Gratings in Multicore Fibers
08:48

Writing Bragg Gratings in Multicore Fibers

Published on: April 20, 2016

8.7K

Area of Science:

  • Food Science
  • Optics
  • Materials Science

Background:

  • Muscle tissue exhibits iridescence due to its photonic structure, specifically well-ordered gratings formed by protruding fibrils.
  • This optical phenomenon can be misinterpreted by consumers as an indicator of product quality.
  • Understanding and quantifying this iridescence is crucial for accurate product assessment.

Purpose of the Study:

  • To explain the fundamentals of iridescence in muscle tissue.
  • To develop a method for quantitatively measuring iridescence caused by diffraction gratings on pork muscle surfaces.
  • To investigate methods for minimizing or removing this optical effect.

Main Methods:

  • Theoretical discussion of iridescence as a light phenomenon.
  • Quantitative measurement of iridescence using reflection spectrophotometry.
  • Real-time monitoring of spectral measurements during controlled drying of muscle tissue gratings.

Main Results:

  • The intensity of diffraction significantly diminishes upon drying the surface gratings.
  • Controlled drying at 50 °C for 2 min reduced diffraction intensity.
  • The diffracted light wavelength was measured at 585 ± 9 nm, suggesting a blazed surface grating.

Conclusions:

  • The study provides a quantitative method to measure iridescence in pork muscle tissue.
  • Findings suggest that diffraction is caused by a blazed surface grating.
  • Guidelines can be developed to alter surface microstructure and minimize iridescence, thereby removing the misleading optical effect.