Histochemical demonstration of Cl(-)-ATPase in rat spinal motoneurons

Using an enzyme-histochemical technique, rat spinal cords were stained for Cl(-)-ATPase, Na+,K+-ATPase and anion-insensitive Mg2+-ATPase. Cl(-)-ATPase activity was demonstrated in plasma membranes of spinal motoneurons, Na+,K+-ATPase activity and anion-insensitive Mg2+-ATPase activity were detected in neuronal plasma membranes and blood vessels, respectively.