Related Experiment Video

Updated: Mar 7, 2026

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles
09:00

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles

Published on: March 18, 2015

12.1K

Arsenic trioxide regulates gastric cancer cell apoptosis by mediating cAMP

A Abudoureyimu1, A Muhemaitibake

  • 1Department of Geriatrics, Xinjiang Medical University Affiliated Hospital of Traditional Chinese Medicine, Urumqi, Xinjiang, China. HongyuZhengzxc@163.com.

European Review for Medical and Pharmacological Sciences
|February 28, 2017
PubMed
Abstract

Insights

Arsenic trioxide (As2O3) promotes apoptosis in gastric cancer cells by increasing cyclic adenosine monophosphate (cAMP) and decreasing protein kinase C (PKC). This study investigates As2O3

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Gastric cancer is a prevalent malignancy with rising incidence.
  • Arsenic trioxide (As2O3) shows promise in inhibiting digestive system tumors.

Purpose of the Study:

  • To investigate the effect of As2O3 on gastric cancer BGC-823 cell apoptosis.
  • To analyze the relationship between As2O3, apoptosis, cyclic adenosine monophosphate (cAMP), and protein kinase C (PKC).

Main Methods:

  • Gastric cancer BGC-823 cells were treated with varying concentrations of As2O3.
  • Apoptosis was assessed using TUNEL assay.
  • Cell cycle, cAMP, PKC, and apoptosis-related proteins were analyzed via flow cytometry, radioimmunoassay, and Western blot.

Main Results:

  • As2O3 significantly increased BGC-823 cell apoptosis.
  • Cell cycle arrest was observed in the G0/G1 phase.
  • cAMP and Bax levels increased, while PKC, Bcl-2, and Survivin expression decreased.

Conclusions:

  • As2O3 induces apoptosis in gastric cancer cells.
  • The mechanism involves up-regulation of cAMP and down-regulation of PKC.

Related Concept Videos

GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
7.9K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.1K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
7.2K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K