Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relationships between behavioral rhythms, plasma corticosterone and hypothalamic circadian rhythms.

M S Kafka1, M A Benedito, L K Steele

  • 1Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, MD 20892.

Chronobiology International
|January 1, 1986
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The MCP-4/MCP-1 ratio in plasma is a candidate circadian biomarker for chronic post-traumatic stress disorder.

Translational psychiatry·2017
Same author

Immunocytochemical study of parvalbumin, calbindin D-28k, and calretinin in the superficial dorsal horn of the rat spinal cord following unilateral hindpaw inflammation.

Journal of anesthesia·2013
Same author

Sympathetic innervation and chemical sympathectomy of canine bladder.

Urology·2011
Same author

Identification of a calcium binding protein highly localized to the cochlear nucleus.

Neurochemistry international·2010
Same author

Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-I. Identification of calmodulin and the b subunit of calcineurin.

Neurochemistry international·2010
Same author

Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-II. analysis of Ca(2+) binding proteins in ammonium sulfate fractions of rat brain.

Neurochemistry international·2010

Circadian rhythms in rat behavior and corticosterone align with hypothalamic norepinephrine (NE) receptor binding and release. Suprachiasmatic nucleus (SCN) arginine vasopressin (AVP) may drive these physiological rhythms.

Area of Science:

  • Neuroscience
  • Chronobiology
  • Physiology

Background:

  • Circadian rhythms govern daily physiological and behavioral cycles.
  • The suprachiasmatic nucleus (SCN) is the master circadian pacemaker.
  • Norepinephrine (NE) and arginine vasopressin (AVP) are key neurochemicals involved in circadian regulation.

Purpose of the Study:

  • To compare circadian rhythms in rat behavior and physiology with hypothalamic neurochemical rhythms.
  • To investigate the role of NE metabolites, adrenergic receptors, and SCN AVP in circadian regulation.

Main Methods:

  • Rats were maintained under constant darkness (D:D) conditions.
  • Circadian rhythms of eating, drinking, locomotor activity, plasma corticosterone, hypothalamic NE metabolites (DHPG, MHPG), adrenergic receptor binding (alpha 1, alpha 2, beta), cAMP, cGMP, and SCN AVP were measured.

Related Experiment Videos

  • Rhythms were analyzed under D:D conditions to assess endogenous circadian properties.

    • Main Results:

    • Behavioral rhythms (activity, eating, drinking) and corticosterone levels exhibited clear circadian patterns.
    • Hypothalamic alpha 1- and beta-adrenergic receptor binding paralleled behavioral rhythms, peaking during subjective night.
    • SCN AVP levels showed a distinct peak in the middle of the subjective day, suggesting a role in pacemaking.
    • Cyclic GMP (cGMP) levels decreased at subjective dusk, potentially releasing nocturnal activity inhibition.
    • No significant circadian rhythms were observed for alpha 2-adrenergic receptor binding, MHPG, or cAMP.

    Conclusions:

    • Circadian rhythms in behavior and corticosterone are closely linked to hypothalamic alpha 1- and beta-adrenergic receptor activity and NE release.
    • SCN AVP rhythmicity may be crucial for the SCN's pacemaking function, influencing downstream circadian outputs.
    • cGMP dynamics suggest a role in modulating activity levels throughout the circadian cycle.