Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through...

Area of Science:

• Agricultural science
• Plant pathology
• Molecular biology

Background:

• Southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) significantly reduce rice grain yield.
• Developing rice cultivars with high resistance to these viruses is essential for food security.

Purpose of the Study:

• To evaluate and identify rice cultivars exhibiting high resistance to SRBSDV and RRSV.
• To investigate the proteomic mechanisms underlying rice resistance to SRBSDV.

Main Methods:

• Field trials were conducted in Yunnan province, China.
• Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect virus gene expression.
• Label-free shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomic analysis was performed.

Main Results:

• Zhongzheyou1 (Z1) and Liangyou2186 (L2186) demonstrated superior resistance, characterized by higher acre yield (A.Y.), lower incidence of infected plants (I.I.P.), virus load (V.L.), disease index (D.I.), and insect quantity (I.Q.).
• Proteomic analysis suggested that systemic acquired resistance (SAR)-related proteins contribute to the enhanced SRBSDV tolerance in Z1 and L2186 compared to Fengyouxiangzhan (FYXZ).

Conclusions:

• Zhongzheyou1 and Liangyou2186 are highly suitable rice cultivars for cultivation in regions affected by SRBSDV and RRSV.
• Understanding the proteomic basis of resistance can aid in breeding more resilient rice varieties.