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Related Concept Videos

The Cochlea01:13

The Cochlea

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The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.
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Related Experiment Video

Updated: Mar 7, 2026

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
09:53

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

Published on: January 1, 2018

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Exploiting decellularized cochleae as scaffolds for inner ear tissue engineering.

Adam J Mellott1, Heather E Shinogle2, Jennifer G Nelson-Brantley3

  • 1Department of Plastic Surgery, University of Kansas Medical Center, Kansas City, KS, 66160, USA.

Stem Cell Research & Therapy
|March 1, 2017
PubMed
Summary
This summary is machine-generated.

Decellularized cochlear tissue can serve as a scaffold for human Wharton

Keywords:
CochleaDecellularizationGene deliveryHair cellsStem cellsTissue engineering

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Area of Science:

  • Tissue Engineering
  • Regenerative Medicine
  • Otolaryngology

Background:

  • Decellularized tissues are valuable scaffolds in tissue engineering, providing physical cues for tissue restoration.
  • The potential of decellularized cochlear tissue as a scaffold for exogenous cell incorporation remains unexplored.
  • Previous research focused on decellularization strategies for various tissues but not specifically the cochlea.

Purpose of the Study:

  • To investigate if decellularized cochlear tissue can support the implantation and survival of exogenous cells.
  • To assess the potential of decellularized cochlear matrix to promote cell differentiation.

Main Methods:

  • Human Wharton's jelly cells were perfused into decellularized mouse cochleae.
  • Decellularization was confirmed using scanning electron microscopy.
  • Cellular presence and differentiation markers (Myosin VIIa) were analyzed using DAPI staining and confocal microscopy.

Main Results:

  • Scanning electron microscopy confirmed acellular structures in decellularized cochleae.
  • Confocal microscopy demonstrated successful implantation and adherence of human Wharton's jelly cells.
  • Some implanted cells expressed Myosin VIIa, indicating potential differentiation.

Conclusions:

  • Decellularized cochlear extracellular matrix can successfully host implanted human Wharton's jelly cells.
  • Myosin VIIa expression suggests the cochlear matrix may influence cell differentiation, a key finding for cochlear tissue engineering.