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Related Experiment Videos

Insulinotropic effects of vanadate.

J A Fagin1, K Ikejiri, S R Levin

  • 1Research Service, Wadsworth VA Hospital, Los Angeles, CA 90073.

Diabetes
|December 1, 1987
PubMed
Summary

Sodium vanadate stimulates insulin secretion from rat islets in a biphasic manner, particularly when extracellular calcium is absent. Its precise mechanism on insulin release remains under investigation.

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Area of Science:

  • Biochemistry
  • Endocrinology
  • Cell Physiology

Background:

  • Vanadium compounds, notably sodium vanadate, are known inhibitors of Na+-K+-ATPase and other phosphoenzymes.
  • Previous research indicated that insulin secretagogues can inhibit rat islet cation-dependent ATPases.

Purpose of the Study:

  • To investigate the effects of sodium vanadate on rat insulin secretion from pancreatic islets.
  • To explore the relationship between vanadate, calcium, glucose, and insulin release.

Main Methods:

  • Incubation and perifusion of rat islets.
  • Measurement of immunoreactive insulin (IRI) secretion.
  • Assay of islet membrane Na+-K+-ATPase activity.
  • Use of calcium, glucose, somatostatin, epinephrine, and diphenylhydantoin as experimental modulators.

Main Results:

  • Sodium vanadate induced biphasic IRI secretion in the presence of 2.4 mM Ca2+ and 100 mg/dl glucose.
  • In the absence of extracellular Ca2+, vanadate significantly increased IRI release at both 100 and 300 mg/dl glucose.
  • The stimulatory effect of vanadate on IRI secretion was abolished by somatostatin, epinephrine, and diphenylhydantoin.
  • Vanadate demonstrated a dose-dependent inhibition of islet membrane Na+-K+-ATPase activity.

Conclusions:

  • Sodium vanadate stimulates insulin secretion from rat islets, with a pronounced effect in low extracellular calcium conditions.
  • While vanadate inhibits islet Na+-K+-ATPase, its exact mechanism for stimulating insulin secretion is complex and likely involves multiple islet phosphoenzymes.
  • Vanadate may serve as a valuable tool for elucidating the intricate mechanisms of insulin secretion.

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