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Breed traceability of buffalo meat using microsatellite genotyping technique
Bheemashankar H Kannur1, Md Nadeem Fairoze2, P S Girish3
1Department of Animal Husbandry and Veterinary Services, Government of Karnataka, Bengaluru, India.
Journal of Food Science and Technology
|March 1, 2017
Summary
This study developed a microsatellite genotyping technique for buffalo breed traceability, successfully identifying buffalo meat origins. This method addresses a research gap, enabling accurate identification of seven Indian buffalo breeds.
Area of Science:
- Animal Genetics
- Zoology
- Biotechnology
Background:
- Buffaloes are significant meat producers in Asia, yet breed traceability research lags behind cattle.
- A knowledge gap exists in understanding buffalo breed origins and genetic diversity.
Purpose of the Study:
- To develop and validate a microsatellite (STR) marker-based technique for Indian buffalo breed traceability.
- To address the lack of genetic identification methods for buffalo meat products.
Main Methods:
- Utilized eight microsatellite (STR) markers across seven Indian buffalo breeds: Bhadawari, Jaffaarabadi, Murrah, Mehsana, Nagpuri, Pandharpuri, and Surti.
- Genotyped six highly polymorphic loci, including CSSMO47, DRB3, and CSSM060, to determine breed-specific profiles.
- Validated the developed technique using known and unknown blood and meat samples.
Main Results:
- The microsatellite genotyping technique successfully traced genetic origins in 24 out of 25 tested samples.
- Identified specific polymorphic markers (CSSMO47, DRB3, CSSM060) crucial for differentiating buffalo breeds.
- Established breed-specific genotypes for seven Indian buffalo breeds.
Conclusions:
- The developed microsatellite genotyping method is effective for buffalo breed traceability, particularly for meat products.
- This research provides a foundation for creating a global archive of buffalo breed-specific genotypes.
- Encourages further research to enhance buffalo traceability systems in Asia and worldwide.
