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Imaging windows for long-term intravital imaging: General overview and technical insights
Maria Alieva1, Laila Ritsma2, Randy J Giedt3
1Cancer Genomics Netherlands; Hubrecht Institute-KNAW and University Medical Centre Utrecht; CT Utrecht, The Netherlands.
Intravital
|March 1, 2017
Summary
This review compares long-term intravital microscopy imaging windows for live animal studies. It offers technical guidance for tracking cells like cancer cells over extended periods.
Area of Science:
- Biomedical Imaging
- Cell Biology
- Cancer Research
Background:
- Intravital microscopy enables visualization of subcellular processes in vivo.
- Long-term cell tracking in live animals requires specialized imaging windows.
- Various organs can be accessed for intravital imaging.
Purpose of the Study:
- To review the development of intravital microscopy imaging windows.
- To compare commonly used long-term imaging windows for cancer biology.
- To provide practical guidance for establishing long-term intravital imaging procedures.
Main Methods:
- Review of existing literature on intravital microscopy imaging windows.
- Comparison of cranial, dorsal skin fold, mammary, and abdominal imaging windows.
- Detailed technical information on surgical procedures and microscopy setups.
Main Results:
- Identified and compared four key long-term imaging windows for cancer research.
- Provided troubleshooting tips and strategies for reproducible imaging sessions.
- Highlighted the importance of imaging window selection for successful long-term studies.
Conclusions:
- Intravital microscopy with appropriate imaging windows is crucial for studying dynamic biological processes.
- This review serves as a resource for researchers establishing long-term intravital imaging.
- Standardized procedures and technical details enhance the reliability of in vivo cell tracking.
