Identification and Quantification of Human Brown Adipose Tissue
Maria Chondronikola1,2, Craig Porter3,4, John O Ogunbileje3,4
1Center for Human Nutrition, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
This chapter details methods for measuring brown adipose tissue (BAT), a key target for combating obesity. Techniques include PET/CT scans, mitochondrial respiration analysis, and UCP1 gene/protein expression measurement.
Area of Science:
- Metabolic research
- Obesity research
- Adipose tissue biology
Background:
- Brown adipose tissue (BAT) is a significant focus for metabolic disease research.
- Understanding and quantifying BAT is crucial for developing anti-obesity strategies.
Purpose of the Study:
- To provide an overview of methods for identifying and quantifying human brown adipose tissue.
- To describe specific procedures for BAT assessment.
Main Methods:
- Positron emission tomography-computed tomography (PET/CT) using 2-deoxy-2-[18F]fluoroglucose (18F-FDG) for in vivo BAT quantification.
- Measurement of mitochondrial respiration.
- Assessment of uncoupling protein 1 (UCP1) gene and protein expression.
Main Results:
- The chapter outlines established methodologies for human BAT assessment.
- Detailed procedural descriptions are provided for each method.
Conclusions:
- Accurate quantification of BAT is achievable through various established techniques.
- These methods are essential for advancing research into obesity and metabolic disorders.
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