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Organization of the human pro-alpha 2(I) collagen gene
W de Wet1, M Bernard, V Benson-Chanda
1Department of Biochemistry, Potchefstroom University, South Africa.
The Journal of Biological Chemistry
|November 25, 1987
Abstract:
Sixty kilobases of cloned DNA containing the entire human pro-alpha 2(I) collagen gene and 22 kilobases of flanking sequences have been isolated. Like the homologous avian gene, the 1366 amino acid residues of the human pre-pro-alpha 2(I) chain are encoded by 52 exons, whose relative locations and sizes have been determined. Analysis of the 5'- and 3'-untranslated regions have confirmed their exact lengths, as well as conclusively established the nature of five polymorphic mRNA transcripts.