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Updated: Mar 7, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

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Silencing Transmembrane Protein 45B (TNEM45B) Inhibits Proliferation, Invasion, and Tumorigenesis in Osteosarcoma

Yan Li, Wei Guo, Shen Liu

    Oncology Research
    |March 1, 2017
    PubMed

    Insights

    Transmembrane protein 45B (TMEM45B) is upregulated in osteosarcoma and drives cancer cell proliferation and migration. Inhibiting TMEM45B suppressed tumor growth, suggesting it acts as an oncogene.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Biochemistry

    Background:

    • Transmembrane protein 45B (TMEM45B) expression is altered in various human tumors.
    • The specific role of TMEM45B in osteosarcoma pathogenesis is not well understood.

    Purpose of the Study:

    • To investigate the biological function of TMEM45B in osteosarcoma.
    • To determine if TMEM45B regulates osteosarcoma cell behavior and progression.

    Main Methods:

    • Quantitative real-time PCR and Western blotting to assess TMEM45B expression.
    • In vitro cell assays (proliferation, migration, invasion) following TMEM45B knockdown.
    • In vivo tumor xenograft model in nude mice to evaluate TMEM45B's effect on tumor growth.
    • Analysis of Wnt/β-catenin signaling pathway components (β-catenin, cyclin D1, c-Myc).

    Main Results:

    • TMEM45B expression was significantly upregulated at both protein and mRNA levels in osteosarcoma cell lines.
    • TMEM45B knockdown inhibited osteosarcoma cell proliferation, migration, and invasion in vitro.
    • Knockdown of TMEM45B led to decreased expression of β-catenin, cyclin D1, and c-Myc.
    • TMEM45B inhibition attenuated tumor growth in vivo.

    Conclusions:

    • TMEM45B plays a crucial role in promoting osteosarcoma cell proliferation, migration, and invasion.
    • The oncogenic effects of TMEM45B in osteosarcoma are partially mediated through the Wnt/β-catenin signaling pathway.
    • TMEM45B is a potential oncogene and therapeutic target in osteosarcoma development and progression.

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