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Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin01:26

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Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
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Consideration of Muscle Depth for Botulinum Toxin Injections: A Three-Dimensional Approach.

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Updated: Mar 7, 2026

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Consideration of Muscle Depth for Botulinum Toxin Injections: A Three-Dimensional Approach.

Julie Bass Kaplan1

  • 1Julie Bass Kaplan, MSN, RN, CPSN, CANS, PHN, HCMT, works for Allergan as an ACE speaker/trainer, Scientiae Palette Resources as a speaker/trainer, and is a regional trainer for CosmoFrance, DermaSculpt MicroCannulas. She is the founder of Disappearing Act Laser & Skin Rejuvenation, Redding, CA. She also owns Allergan stock.

Plastic Surgical Nursing : Official Journal of the American Society of Plastic and Reconstructive Surgical Nurses
|March 1, 2017
PubMed
Summary
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Understanding facial muscle anatomy, including depth and attachments, is crucial for safe and effective botulinum toxin injections. A 3D approach enhances aesthetic outcomes by avoiding common injection errors.

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Area of Science:

  • Anatomy
  • Medical Aesthetics
  • Dermatology

Background:

  • Accurate administration of botulinum toxin for aesthetic purposes relies on comprehensive knowledge of facial anatomy.
  • Variable muscle depth and complex overlapping planes can lead to suboptimal results or adverse effects if overlooked.
  • Traditional 2D anatomical approaches may not fully prepare injectors for the nuances of facial muscle injection.

Purpose of the Study:

  • To explore a three-dimensional (3D) anatomical approach for botulinum toxin administration in aesthetic medicine.
  • To highlight the importance of understanding muscle depth, origin, and attachment for precise injections.
  • To identify key facial muscles for injection and those to avoid for optimal aesthetic outcomes.

Main Methods:

  • Review of anatomical literature focusing on facial muscles relevant to aesthetic injections.
  • Discussion of a 3D anatomical perspective, contrasting with traditional 2D methods.
  • Outline of twelve commonly injected facial muscles and seven to generally avoid.

Main Results:

  • A 3D anatomical understanding allows for prediction of patient outcomes and avoidance of injection errors.
  • Variability in muscle depth, such as the corrugator supercilii, requires careful consideration.
  • Incorrect injections can result in undesirable aesthetic effects like a heavy brow or angry appearance.

Conclusions:

  • A 3D anatomical approach is superior to 2D methods for achieving excellent outcomes with botulinum toxin.
  • Knowledge of facial muscle variability, depth, and attachments is essential for all injectors.
  • The article provides guidance on muscles to target and avoid for safe and effective aesthetic treatments.