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Updated: Mar 7, 2026

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
Published on: December 16, 2022
Highly efficient RNA-guided base editing in mouse embryos
Kyoungmi Kim1, Seuk-Min Ryu1,2, Sang-Tae Kim1
1Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea.
Abstract:
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.
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