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Fruit Development, Structure, and Function01:58

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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development.

Dong Liang1, Tingting Zhu2, Zhiyou Ni2

  • 1Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.

Plos One
|March 1, 2017
PubMed
Summary
This summary is machine-generated.

Ascorbic acid (AsA) concentration in sweet cherries peaks early and declines during ripening, but total fruit accumulation increases due to growth. Key genes regulating AsA metabolism coordinate its levels during fruit development.

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Fruit Development

Background:

  • Ascorbic acid (AsA), also known as Vitamin C, is a crucial antioxidant in plants.
  • Understanding AsA metabolism is vital for improving fruit quality and nutritional value.

Purpose of the Study:

  • To investigate the metabolic pathways and gene expression related to ascorbic acid in sweet cherry fruit ('Hongdeng').
  • To correlate AsA concentration patterns with the expression of genes and enzyme activities during fruit development and maturation.

Main Methods:

  • Quantification of AsA concentration throughout sweet cherry fruit development.
  • Cloning of cDNAs for genes involved in AsA biosynthesis and recycling pathways.
  • Analysis of mRNA expression levels and enzyme activities of key genes in AsA metabolism.

Main Results:

  • AsA concentration was highest at petal-fall and decreased during ripening, with a slight rise at maturity.
  • Despite concentration changes, total AsA accumulation increased with fruit fresh weight.
  • Expression patterns of genes like GGP2, GalLDH, APX3, AO2, GR1, and DHAR1 correlated with AsA concentration dynamics.

Conclusions:

  • Genes involved in ascorbic acid biosynthesis, degradation, and recycling act in concert to regulate AsA accumulation in sweet cherry fruit.
  • The interplay between gene expression and enzyme activity is critical for managing AsA levels during fruit development.