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[Establishment and Identification of SCL-tTA/BCR-ABL Transgenic Mouse Model with Chronic Myeloid Leukemia]
Yan Lin1, Wei Fu2, Ying-Min Liang3
1Department of Pediatrics, Tangdu Hospital of The Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China.
Objective:
To establish transgenic mouse model with chronic myeloid leukemia(CML) by tetracycline withdrawal, so as to provide the experimental tool for studying pathogenesis of CML and searching new therapeutic method for this disease.
Methods:
The BCR-ABL expression in stem and progenitor cells in SCL-tTA/BCR-ABL transgenic mice was induced by tetracycline withdrawal in drinking water; and the peripheral hemogram and spleen index were examined after identifying genotypes in mice; the distribution of hematopoietic lineage in bone marrow, spleen and peripheral blood was detected by flow cytometry.
Results:
As compared with the control mice, the peripheral hemogram showed the obvious increase of neutrophile granulocyte count and spleen index in BCR-ABL transgenic mice. All induced double transgenic mice showed a severe myeloid cell expansion (Gr-1+Mac-1+) and reduction in T (CD3+) and B(B220+) cell lineages in bone marrow, spleen and peripheral blood.
Conclusion:
A mouse model to induce BCR-ABL expression is successfully set up by tetracycline withdrawal, which resembles chronic phase CML.
