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[Effect of Icaritin on Proliferation and Apoptosis of THP-1 Cell and Its Mechanism]
Wen-Yong Kuang1, Min-Cui Zheng1, Guang-Sen Zhang2
1Department of Hematology,Hunan Provincial Children's Hospital,Changsha 410007,Hunan Province,China.
Objective:
To investigate the effect of icaritin on the proliferation and apoptosis of THP-1 cells and its mechanism.
Methods:
After treated with various concentrations of icaritin, cell proliferation was detected by MTS method, and apoptosis was measured with flow cytometry and Hoechst 33258 staining. Expression of BCL-2, BAX and Caspase-3 protein in THP-1 cell was detected by Western blot.
Results:
After treatment with various concentrations (4-32 µmol/L) of icaritin for 24, 48, 72 h, the inhibition rate of cell growth significantly increased (P<0.05) in time-dose dependent manner(r=0.946); and the apoptotic rate of cells significantly increased (P<0.05) in time-dose dependent manner(r= 0.924). The expression of BCL-2 protein at 48 h decreased significantly in icaritin-treated group, compared with that in control group (P<0.05), while the expression of BAX and Caspase3 protein at 48 h increased significantly in icaritin-treated group, compared with that in control group (P<0.05).
Conclusion:
Icaritin can inhibit proliferation and induce apoptosis of THP-1 in vitro, Icaritin may induce apoptosis in THP-1 cells through the mitochondrial pathway.
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