Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Role of Amputation and Myoelectric Prosthetic Fitting in Traumatic Adult Brachial Plexus Injury.

Hand clinics·2026
Same author

Glenohumeral arthrodesis improves elbow flexion in patients with adult brachial plexus injuries.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2026
Same author

Nonbrachial Plexopathy Diagnoses Seen in a Brachial Plexus Injury Specialty Clinic.

Journal of hand surgery global online·2026
Same author

A Clean Slate.

Techniques in hand & upper extremity surgery·2026
Same author

Response to "Letter Regarding 'Ten Reasons Why Prospective Randomized Studies in Surgery Are Flawed and Fundamentally Different From Drug Trials'".

The Journal of hand surgery·2026
Same author

Differentiating Upper Trunk Brachial Plexopathies From the Electrodiagnostic "Gray Zone" Extraforaminal Cervical Nerve Root Injuries With Brachial Plexus Ultrasound: A case series.

American journal of physical medicine & rehabilitation·2026

Related Experiment Video

Updated: Mar 7, 2026

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
08:57

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

Published on: February 10, 2023

2.8K

Optimizing decellularization techniques to create a new nerve allograft: an in vitro study using rodent nerve

Caroline A Hundepool1,2, Tim H J Nijhuis2, Dimitra Kotsougiani1

  • 1Department of Orthopedic Surgery, Microvascular Research Laboratory, Mayo Clinic, Rochester, Minnesota; and.

Neurosurgical Focus
|March 2, 2017
PubMed
Summary

Optimizing nerve allografts using elastase processing and cold storage significantly reduced immunogenicity and cellular debris while preserving ultrastructure. Frozen storage at -80°C, however, damaged nerve structure.

Keywords:
BL = basal laminaMHCI = major histocompatibility complex–ISB-16 = sulfobetaine-16decellularizationelastasenerve allograftprocessing

More Related Videos

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury
10:33

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Published on: February 23, 2014

12.3K
Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
09:09

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

Published on: July 12, 2022

5.3K

Related Experiment Videos

Last Updated: Mar 7, 2026

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
08:57

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

Published on: February 10, 2023

2.8K
Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury
10:33

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Published on: February 23, 2014

12.3K
Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
09:09

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

Published on: July 12, 2022

5.3K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Commercially processed nerve allografts show inferior outcomes compared to autografts in animal models.
  • Optimizing processing and storage is crucial for improving nerve allograft efficacy.

Purpose of the Study:

  • To develop an optimized nerve allograft by evaluating different processing and storage techniques.
  • To enhance ultrastructure preservation, reduce immunogenicity, and minimize cellular debris in nerve allografts.

Main Methods:

  • Rat nerves underwent decellularization using modified protocols including elastase.
  • Nerve segments were stored at either 4°C (cold) or -80°C (frozen).
  • Analysis included confocal microscopy, immunohistochemistry (laminin γ-1, S100, MHCI), and electron microscopy.

Main Results:

  • All decellularization protocols preserved nerve ultrastructure.
  • Storage at -80°C significantly damaged nerve ultrastructure post-decellularization.
  • Elastase treatment reduced immunogenicity and Schwann cell presence while maintaining structural integrity.

Conclusions:

  • Elastase addition to nerve processing reduces immunogenicity and cellular debris, preserving ultrastructure.
  • Cold storage (4°C) is superior to frozen storage (-80°C) for maintaining nerve allograft ultrastructure.
  • Further in vivo studies are necessary to confirm the regenerative potential of these optimized nerve allografts.