Related Experiment Video

Updated: Mar 7, 2026

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
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Published on: June 28, 2013

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A Comprehensive TALEN-Based Knockout Library for Generating Human-Induced Pluripotent Stem Cell-Based Models for

Ioannis Karakikes1, Vittavat Termglinchan1, Diana A Cepeda1

  • 1From the Stanford Cardiovascular Institute (I.K., V.T., J.L., S.D., I.I., M.A., R.S., H.W., N.M., N.-Y.S., T.S., N.W., K.D.W., E.M., J.C.W.), Department of Cardiothoracic Surgery (I.K.), Division of Cardiovascular Medicine, Department of Medicine (V.T., J.C.W.), CA; Institute of Stem Cell Biology and Regenerative Medicine (D.A.C., V.S., J.C.W.), Departments of Pediatrics (A.H., M.H.P.), Pathology (K.D.W.), and Obstetrics and Gynecology (V.S.), Stanford University School of Medicine, CA; Berlin Institute of Health, Germany (S.D.); and Max Delbrueck Center, Berlin, Germany (S.D.).

Circulation Research
|March 2, 2017
PubMed
Summary

This study utilized transcription activator-like effector nucleases (TALENs) and human induced pluripotent stem cells (iPSCs) to create gene knockouts for studying cardiovascular diseases. The developed methods offer a valuable resource for cardiovascular research.

Keywords:
dilated cardiomyopathygene knockoutgene targetinggenome editingstem cell

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