Phosphoproteome Discovery in Human Biological Fluids

Area of Science:

• Biochemistry
• Proteomics
• Analytical Chemistry

Background:

• Protein phosphorylation is a key regulator of cellular processes.
• Global phosphoproteomics is a rapidly advancing research field.
• Phosphoproteins in biological fluids offer diagnostic and therapeutic potential.

Purpose of the Study:

• To review phosphoproteome discovery in human biological fluids.
• To discuss bioanalytical workflows, particularly mass spectrometry-based approaches.
• To summarize existing studies on phosphoproteome discovery in major fluids.

Main Methods:

• Focus on mass spectrometry-based techniques for phosphoproteomics.
• Discussion of bioanalytical workflows tailored for biological fluids.
• Compilation and summary of published phosphoproteome studies.

Main Results:

• Phosphoproteome analysis in serum/plasma, urine, CSF, saliva, and BAL fluid is feasible.
• Mass spectrometry enables global phosphoprotein identification.
• Studies reveal the potential of phosphoproteomics for understanding disease mechanisms.

Conclusions:

• Phosphoproteomics of biological fluids is a promising area for biomarker discovery.
• Advancements in analytical technologies are crucial for clinical translation.
• Further research can enhance understanding of protein function in health and disease.