Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Statistical insights into the role of HCN-mineral interactions in aqueous aerosols during chemical evolution.

Communications chemistry·2026
Same author

Grapefruit By-Products as a Sustainable Source of Bioaccessible Polyphenols with In Vitro Neuroprotective Potential.

International journal of molecular sciences·2026
Same author

Development of New Red-Fleshed Seedless Table Grapes: In Vitro Insights on Glucose Absorption and Insulin Resistance Biomarkers.

Foods (Basel, Switzerland)·2025
Same author

Human corneal epithelial cells harvested from advanced surface ablation (ASA): An optimized in vitro culture protocol.

Experimental eye research·2025
Same author

Effective ocular delivery of antioxidant polyphenols using elastin-like polymer nanosystems developed by sustainable process.

International journal of pharmaceutics·2025
Same author

<i>Ex vivo</i> applications of porcine ocular surface tissues: Advancing eye research and alternatives to animal studies.

Histology and histopathology·2025

Related Experiment Video

Updated: Mar 7, 2026

Induction of Ocular Surface Inflammation and Collection of Involved Tissues
06:38

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

3.0K

An engineered human conjunctival-like tissue to study ocular surface inflammatory diseases.

Laura García-Posadas1,2, Laura Soriano-Romaní1,2, Antonio López-García1,2

  • 1Ocular Surface Group, Institute for Applied Ophthalmobiology (IOBA), University of Valladolid, Valladolid, Spain.

Plos One
|March 2, 2017
PubMed
Summary

Researchers developed a 3D human conjunctiva model using fibrin scaffolds to study ocular surface diseases. This model successfully mimics conditions like dry eye and allergies, aiding research into inflammatory eye conditions.

More Related Videos

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
07:35

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

10.9K
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

9.3K

Related Experiment Videos

Last Updated: Mar 7, 2026

Induction of Ocular Surface Inflammation and Collection of Involved Tissues
06:38

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

3.0K
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
07:35

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

10.9K
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

9.3K

Area of Science:

  • Ocular surface research
  • Tissue engineering
  • Cell biology

Background:

  • The human conjunctiva plays a crucial role in ocular surface health.
  • Existing models lack the complexity to fully replicate conjunctival physiology and pathophysiology.
  • A need exists for advanced in vitro models to study inflammatory eye diseases.

Purpose of the Study:

  • To develop a functional three-dimensional (3D) model of the human conjunctiva.
  • To utilize this model for experiments simulating ocular surface physiology and disease states.
  • To investigate the effects of specific conditions on conjunctival constructs.

Main Methods:

  • Development of fibrin-based scaffolds from human plasma or cryoprecipitate.
  • Isolation and culture of primary human conjunctival cells on scaffolds.
  • Analysis using immunofluorescence, scanning electron microscopy, alamarBlue® assay, H&E staining, HPA staining, and ELISA for MUC5AC and IL-6.

Main Results:

  • Fibrin scaffolds supported conjunctival cell growth, stratification, and polarization, forming functional microvilli.
  • Air-lifted, partially desiccated, and IL-13-treated cultures showed increased MUC5AC secretion.
  • Elevated IL-6 levels indicated inflammation in air-lifted and desiccated cultures, but not in IL-13-treated ones.

Conclusions:

  • A novel 3D human conjunctiva model was successfully developed using fibrin matrices.
  • This model supports cell growth and stratification, mimicking key conjunctival functions.
  • The model effectively replicates aspects of dry eye disease and allergic responses, enabling the study of ocular surface inflammatory conditions.