Rapid fabrication of silver nanoparticle-coated filter paper as SERS substrate for low-abundance molecules detection

Silver nanoparticles (Ag NPs) were fabricated on the fibers of the filter paper by the reaction between silver nitrate (AgNO₃) and hydrazine hydrate (N₂H₄·H₂O). By using the Ag NPs-coated paper, the limit of detection as low as 10^-11M for Rhodamine B (RhB) and 10^-10M for crystal violet (CV) was achieved. Moreover, the uniformity, reproducibility and stability of the Ag NPs-coated paper were also involved. Meanwhile, the detection of 10^-4-10^-6M moxifloxacin in deionized water and tap water was also carried out successfully by using the paper-based substrates. The fabrication process is easy to handle, cost-efficient and the as-prepared paper-based SERS substrate is ideal for rapid and simple detection of low-abundance molecules.