Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The perchlorate discharge test for examining thyroid function in rats.

C K Atterwill1, P Collins, C G Brown

  • 1Department of Specialised Toxicology, Smith Kline & French Research Ltd., Herts, United Kingdom.

Journal of Pharmacological Methods
|November 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevention of Wastage in Convalescent Depots.

War medicine (Paris, France)·2023
Same author

Experimental strategies to discover and develop the next generation of psychedelics and entactogens as medicines.

Neuropharmacology·2022
Same author

A compact filtered x-ray diode array spectrometer for the National Ignition Facility: SENTINEL.

The Review of scientific instruments·2020
Same author

Transvenous Versus Epicardial Pacing in Fontan Patients.

Pediatric cardiology·2018
Same author

X-ray transport and radiation response assessment (XTRRA) experiments at the National Ignition Facility.

The Review of scientific instruments·2016
Same author

Advances in x-ray framing cameras at the National Ignition Facility to improve quantitative precision in x-ray imaging.

The Review of scientific instruments·2016
Same journal

Enhanced detection of hyperactivity after drug withdrawal with a simple modification of the open-field apparatus.

Journal of pharmacological methods·1991
Same journal

Validation of a human atrial trabecular preparation for evaluation of inotropic substances.

Journal of pharmacological methods·1991
Same journal

A method for maintaining and protecting chronic arterial and venous catheters in conscious rats.

Journal of pharmacological methods·1991
Same journal

Quantitation of urinary alpha 2u-globulin and albumin by reverse-phase high performance liquid chromatography.

Journal of pharmacological methods·1991
Same journal

Relative potencies of 5-lipoxygenase inhibitors on antigen-induced contractions of guinea pig tracheal strips.

Journal of pharmacological methods·1991
Same journal

Rabbit aortic smooth muscle cell culture. A model for the pharmacological study of diabetes-induced alterations in cell proliferation.

Journal of pharmacological methods·1991
See all related articles

A new perchlorate discharge test in rats effectively distinguishes between thyroid iodide accumulation and organification issues. This method aids in understanding thyroid function disruptions caused by specific drug treatments.

Area of Science:

  • Endocrinology
  • Pharmacology
  • Thyroid Research

Background:

  • Thyroid function relies on iodide accumulation and organification.
  • Disruptions in these processes can lead to various thyroid disorders.
  • Accurate methods are needed to differentiate between these specific mechanisms.

Purpose of the Study:

  • To develop and validate a perchlorate discharge test in rats.
  • To assess the test's ability to detect alterations in thyroidal iodide handling.
  • To differentiate between impaired iodide accumulation and organification.

Main Methods:

  • Rats were pretreated with SK&F 93479 (thyroid hormone clearance enhancer) or propylthiouracil (iodide organification inhibitor).
  • Radioactive iodine (125I) was administered, followed by potassium perchlorate or saline.

Related Experiment Videos

  • Thyroid:blood 125I ratios were measured to assess iodide trapping and organification.

    • Main Results:

    • Potassium perchlorate significantly reduced the thyroid:blood 125I ratio in propylthiouracil-treated rats, indicating an iodide organification block.
    • Perchlorate had no effect in SK&F 93479-treated rats, suggesting intact organification.
    • Thyroidal radioiodide accumulation was enhanced in SK&F 93479-treated rats and depressed in propylthiouracil-treated rats.

    Conclusions:

    • The perchlorate discharge test effectively distinguishes between impaired iodide organification and altered iodide accumulation in rats.
    • This method provides a valuable tool for studying thyroid pathophysiology.
    • The findings highlight the distinct effects of SK&F 93479 and propylthiouracil on thyroid iodide metabolism.