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Published on: September 22, 2020
Circulating mircoRNA-21 as a predictor for vascular restenosis after interventional therapy in patients with lower
Bo Zhang1, Ye Yao1, Qing-Feng Sun2
1Department of Vascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Insights
Circulating microRNA-21 (miR-21) is elevated in patients with lower extremity arterial occlusive disease (LEAOD) who experience vascular restenosis after interventional therapy. miR-21 shows high accuracy as a predictive biomarker for restenosis in these patients.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Research
Background:
- Vascular restenosis is a significant complication following interventional therapy for lower extremity arterial occlusive disease (LEAOD).
- Identifying reliable biomarkers for predicting restenosis is crucial for patient management and therapeutic strategy development.
Purpose of the Study:
- To investigate the diagnostic and predictive role of circulating microRNA-21 (miR-21) in vascular restenosis among LEAOD patients undergoing interventional treatment.
- To evaluate the association between miR-21 expression levels and clinical characteristics in LEAOD patients with restenosis.
Main Methods:
- A cohort of 412 LEAOD patients was analyzed, categorized into restenosis and non-restenosis groups based on CTA and ABI.
- Circulating miR-21 levels were quantified using quantitative real-time PCR (qRT-PCR) pre- and post-intervention.
- Receiver operating characteristic (ROC) curve analysis was employed to assess the predictive performance of miR-21.
Main Results:
- Patients in the restenosis group were older and had higher incidences of diabetes and hypertension compared to the non-restenosis group.
- Circulating miR-21 expression was significantly higher in LEAOD patients with vascular restenosis.
- miR-21 demonstrated a high predictive value for vascular restenosis with an AUC of 0.938, high sensitivity (83.5%), and specificity (98.2%).
Conclusions:
- Circulating miR-21 is a promising predictive biomarker for vascular restenosis in LEAOD patients after interventional therapy.
- Diabetes and elevated miR-21 expression are identified as major risk factors for vascular restenosis in this patient population.
Abstract:
The present study was designed to investigate the role of circulating miRNA-21 (miR-21) in vascular restenosis of lower extremity arterial occlusive disease (LEAOD) patients after interventional therapy. A total of 412 LEAOD patients were enrolled randomly in the present study. According to computed tomography angiography (CTA) and ankle-brachial index (ABI), patients were assigned into the restenosis group and the non-restenosis group. miR-21 expression was detected with quantitative real-time PCR (qRT-PCR) before and after patients underwent interventional therapy. A follow-up period of 6 months was achieved. A receiver operating characteristic (ROC) curve was drawn and the area under the curve (AUC) was calculated to assess the predictive value of miR-21 in vascular restenosis. Patients were older in the restenosis group than in the non-restenosis group. The percentages of patients with diabetes and hypertension were higher in the restenosis group than in the non-restenosis group, and the Fontaine stage exhibited a significant difference between the two groups. miR-21 expression was higher in the restenosis group than in the non-restenosis group. miR-21 expression level was related to age, diabetes and hypertension in the restenosis group. Using miR-21 to predict vascular restenosis yielded an AUC of 0.938 (95% confidence interval (CI): 0.898-0.977), with Youden index of 0.817, sensitivity of 83.5% and specificity of 98.2%. Logistic regression analysis revealed that diabetes and miR-21 expression were the major risk factors for vascular restenosis of LEAOD. miR-21 can be used as a predictive indicator for vascular restenosis of LEAOD after interventional therapy.
