Related Experiment Video
Updated: Feb 10, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome
1Daniel Rutherford Building G.24, School of Biological Sciences, University of Edinburgh, The King's Buildings, Edinburgh EH9 3BF, United Kingdom; yizhi.cai@ed.ac.uk jbdai@tsinghua.edu.cn.
Abstract:
De novo DNA synthesis is one of the key enabling technologies for synthetic biology. Methods for large-scale DNA synthesis, in particular, have transformed many facets of life science research, supporting new discoveries in biology through the design of novel synthetic biological systems. This protocol describes in detail the methods currently being used to synthesize and assemble large pieces of DNA for the synthetic yeast genome project. The protocol includes instructions for building block synthesis as well as chunk assembly, each of which can be used as a stand-alone procedure to generate a synthetic DNA of interest.
Related Concept Videos
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic DNA in Eukaryotes
Genomics
Yeast Signaling
DNA Replication
Replication in Prokaryotes
DNA replication...
Habitat Fragmentation

