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Updated: Mar 7, 2026
A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
Published on: September 28, 2019
Three-dimensional histology: new visual approaches to morphological changes during neural regeneration
Hei Ming Lai1, Ho Man Ng1, Wutian Wu2
1School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.
Abstract:
Three-dimensional (3D) histology utilizes tissue clearing techniques to turn intact tissues transparent, allowing rapid interrogation of tissue architecture in three dimensions. In this article, we summarized the available tissue clearing methods and classified them according to their physicochemical principles of operation, which provided a framework for one to choose the best techniques for various research settings. Recent attempts in addressing various questions regarding the degenerating and regenerating nervous system have been promising with the use of 3D histological techniques.
