Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

26.5K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.5K
Determination of Crystal Structures01:29

Determination of Crystal Structures

20
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
20
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.0K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Signal propagation in LOV-based multidomain proteins: time-resolved infrared spectroscopy reveals the complete photocycle of YF1 and PAL.

Physical chemistry chemical physics : PCCP·2026
Same author

Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography.

Nature communications·2025
Same author

Tumor Biomechanics Quantified Using MR Elastography to Predict Response to Neoadjuvant Chemotherapy in Individuals with Breast Cancer.

Radiology. Imaging cancer·2025
Same author

Structural Insights Into the Opening Mechanism of C1C2 Channelrhodopsin.

Journal of the American Chemical Society·2024
Same author

Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.

Nature communications·2024
Same author

Kinetic Basis for the Design of Azobenzene-Based Photoswitchable A<sub>2a</sub> Adenosine Receptor Ligands.

Journal of the American Chemical Society·2024

Related Experiment Video

Updated: Mar 6, 2026

Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.9K

Serial crystallography at synchrotrons and X-ray lasers.

Jörg Standfuss1, John Spence2

  • 1Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institute, Villigen PSI, Switzerland.

Iucrj
|March 3, 2017
PubMed
Summary

Serial crystallography, initially for free-electron lasers, now works with synchrotron sources. This synergy expands its use in microcrystallography, room-temperature, and time-resolved studies.

Keywords:
X-ray laserseditorialserial crystallographysynchrotrons

More Related Videos

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

3.7K
Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.6K

Related Experiment Videos

Last Updated: Mar 6, 2026

Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.9K
Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

3.7K
Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.6K

Area of Science:

  • Structural Biology
  • Crystallography
  • Biophysics

Background:

  • Serial crystallography was initially developed for free-electron laser (FEL) sources.
  • The technique has recently been adapted for use with synchrotron X-ray sources.

Discussion:

  • This discussion explores the synergistic potential of combining FEL and synchrotron sources for serial crystallography.
  • The integration of these sources enhances the applicability of serial crystallography across various research domains.

Key Insights:

  • Synergy between FEL and synchrotron sources broadens the application of serial crystallography.
  • The technique facilitates microcrystallography, enabling structure determination from small or limited crystal samples.
  • Enables room-temperature structure determination, preserving native biological states.
  • Supports time-resolved studies, capturing dynamic processes in biological molecules.

Outlook:

  • Future applications of serial crystallography will benefit from the combined strengths of FEL and synchrotron sources.
  • This integrated approach is expected to accelerate discoveries in structural biology and drug development.