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Differential decrease in soluble and DNA-bound telomerase in senescent human fibroblasts.

Snir Yehuda1, Hagai Yanai1, Esther Priel1

  • 1The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, 8410501, Beer Sheva, Israel.

Biogerontology
|March 3, 2017
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Summary
This summary is machine-generated.

Cellular senescence (CS) involves telomere shortening, but the role of telomerase reverse transcriptase (TERT) in normal cells is less understood. This study found TERT levels and activity decrease in senescent human dermal fibroblasts, suggesting non-telomere roles.

Keywords:
Cellular senescenceDermal fibroblastsPrimary culturesTelomerase

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Aging Research

Background:

  • Replicative cellular senescence (CS) is linked to telomere shortening.
  • The role of telomerase reverse transcriptase (TERT) in CS is primarily studied in cancer/immortalized cells.
  • Limited research exists on TERT's function in normal cell senescence.

Purpose of the Study:

  • Investigate TERT expression and telomerase activity in primary adult human dermal fibroblasts (HDFs) undergoing replicative CS.
  • Determine if TERT plays a role in the senescence of normal somatic cells.
  • Explore potential non-canonical functions of TERT in cellular aging.

Main Methods:

  • Primary cultures of adult human dermal fibroblasts (HDFs) were used.
  • An in vitro model of replicative cellular senescence was established.
  • TERT protein levels and telomerase activity were measured in senescent and non-senescent HDFs.
  • TERT distribution in soluble and DNA-bound fractions was analyzed.

Main Results:

  • Active TERT was expressed in HDFs.
  • TERT protein levels and telomerase activity significantly decreased in senescent HDFs.
  • The reduction in TERT was more pronounced in the soluble fraction compared to the DNA-bound fraction.

Conclusions:

  • TERT is expressed in normal human dermal fibroblasts.
  • TERT levels and activity decline during replicative cellular senescence in HDFs.
  • These findings suggest TERT has important non-telomere-related functions in cellular senescence.