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Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
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A 13-week research-based biochemistry laboratory curriculum.

Scott T Lefurgy1, Emily C Mundorff1

  • 1Department of Chemistry, Hofstra University, 151 Hofstra University, Hempstead, NY, 11549.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
|March 3, 2017
PubMed
Summary

This curriculum uses directed enzyme evolution to teach biochemistry lab techniques. Students engage in original research, creating novel enzyme variants and analyzing their function.

Keywords:
Biochemistry laboratory curriculumdirected evolutionresearch-based curriculumundergraduate research

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Traditional biochemistry curricula often lack opportunities for original research.
  • Students benefit from hands-on experience with advanced laboratory techniques.
  • Directed evolution offers a powerful approach to studying enzyme function and engineering.

Purpose of the Study:

  • To present a 13-week research-based biochemistry laboratory curriculum.
  • To introduce foundational biochemistry laboratory techniques through original research.
  • To facilitate student engagement in directed enzyme evolution and analysis.

Main Methods:

  • Directed evolution of instructor-chosen enzymes with student-designed mutations.
  • Saturation mutagenesis and high-throughput screening for enzyme function.
  • Bioinformatics, homology modeling, structural analysis, protein expression/purification, PAGE, UV/Vis spectroscopy, and enzyme kinetics.

Main Results:

  • Successful implementation of student-designed mutant libraries and novel enzyme variants.
  • Generation of original student hypotheses and data, potentially leading to publication.
  • Demonstrated feasibility of integrating original research into undergraduate biochemistry labs.

Conclusions:

  • The curriculum successfully integrates original research and foundational techniques.
  • Students gain experience in advanced methods and scientific inquiry.
  • This approach can foster data quality and potentially lead to novel scientific discoveries.