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Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an
R L Charlebois1, W L Lam, S W Cline
1Department of Biochemistry, Dalhousie University, Halifax, NS, Canada.
Abstract:
We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.