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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

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Evolution of complexity in the zebrafish synapse proteome.

Àlex Bayés^1,2, Mark O Collins³, Rita Reig-Viader^1,2

¹Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Maria Claret 167, 08025 Barcelona, Spain.

Nature Communications

|March 3, 2017

Summary

Zebrafish synapse proteomes were characterized, revealing the teleost-specific genome duplication (TSGD) increases complexity. Despite conserved proteins and structures, zebrafish postsynaptic density proteomes are less complex than mammals, impacting disease modeling.

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Area of Science:

Neuroscience
Proteomics
Evolutionary Biology

Background:

Human brain synapse complexity is linked to over 130 diseases.
Vertebrate genome complexity arose from early whole-genome duplications.
Zebrafish are crucial models for human diseases, but their synapse proteome remains uncharacterized.

Purpose of the Study:

To characterize the proteome and ultrastructure of zebrafish central synapses.
To investigate the influence of the teleost-specific genome duplication (TSGD) on synapse complexity.
To assess the implications for using zebrafish in modeling human synaptic diseases.

Main Methods:

Proteomic analysis of zebrafish central synapses.
Ultrastructural examination of synaptic components.
Comparative analysis with mammalian synapse proteomes.

Main Results:

The TSGD significantly increased overall synapse proteome complexity in zebrafish.
Zebrafish postsynaptic density (PSD) proteomes exhibit lower complexity compared to mammals.
Approximately 1,000 proteins are conserved across vertebrate synapses, with conserved PSD ultrastructure.
Lineage-specific proteome variations suggest distinct evolution of synapse types and functions.

Conclusions:

Zebrafish synapse characterization provides a valuable resource for neuroscience research.
Understanding TSGD's role in synapse complexity is crucial for interpreting zebrafish disease models.
Comparative data highlights conserved and divergent aspects of synaptic evolution across vertebrates.