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Spatiotemporal Control of Supramolecular Self-Assembly and Function.

Jie Zhan1,2, Yanbin Cai1, Shenglu Ji1

  • 1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.

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Summary

Temperature control of enzyme-triggered peptide self-assembly allows for spatiotemporal manipulation. This method produces distinct nanostructures, like nanoparticles and nanofibers, with tunable cellular uptake for advanced nanoprobes.

Keywords:
diffusionenzymekineticsmolecular probepeptideself-assembly

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Enzyme-triggered peptide self-assembly offers precise control over nanostructure formation.
  • The influence of diffusion and chemical gradients on enzyme-generated peptide self-assembly remains underexplored.

Purpose of the Study:

  • To demonstrate spatiotemporal control over enzyme-triggered peptide self-assembly.
  • To investigate the effect of temperature on enzyme activity and peptide aggregation.
  • To produce nanostructures with tunable morphology and cellular uptake.

Main Methods:

  • Utilized temperature adjustments to modulate phosphatase enzyme activity and peptide aggregation states.
  • Controlled peptide production rate and spatial distribution via temperature.
  • Characterized resulting nanostructures (nanoparticles and nanofibers) and their cellular uptake.

Main Results:

  • Achieved distinct nanostructures: nanoparticles at 37 °C and nanofibers at 4 °C.
  • Demonstrated temperature-dependent control over self-assembly kinetics and morphology.
  • Nanofibers exhibited approximately 10-fold higher cellular uptake by 3T3 cells compared to nanoparticles due to enhanced stability and order.

Conclusions:

  • Spatiotemporal control of enzyme-triggered peptide self-assembly is achievable by temperature modulation.
  • This strategy enables the creation of optimized nanoprobes with improved cellular uptake and sensitivity.
  • The approach holds potential for the general fabrication of self-assembled nanomaterials with tailored morphology and function.