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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Mar 6, 2026

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

Published on: March 21, 2018

Comparison of preprocessing methods and storage times for touch DNA samples.

Hui Dong, Jing Wang, Tao Zhang

¹Cai-xia Li, Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing100038, China, licaixia@tsinghua.org.cn.

Croatian Medical Journal

|March 3, 2017

Summary

Selecting the right touch DNA (deoxyribonucleic acid) preprocessing method is crucial for different substrates. This study found direct cutting works best for porous surfaces, while vacuuming is optimal for non-porous ones.

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Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

Touch DNA analysis is vital for forensic investigations.
Optimizing DNA recovery from various substrates is challenging.
Standardized preprocessing methods are needed for reliable touch DNA analysis.

Purpose of the Study:

To evaluate four distinct preprocessing techniques for touch DNA samples.
To determine the efficacy of these methods across different substrate types (porous vs. non-porous).
To assess the impact of sample storage duration on DNA analysis outcomes.

Main Methods:

Investigated touch DNA recovery from mock samples on diverse substrates.
Compared four preprocessing methods: direct cutting, stubbing, double swab, and vacuuming.
Quantified and amplified extracted DNA using standard forensic protocols.

Main Results:

Porous substrates yielded higher DNA amounts and allele numbers than non-porous ones.
The direct cutting method excelled on porous substrates; vacuuming was superior for non-porous substrates.
No significant DNA degradation was observed with increased storage times.

Conclusions:

Substrate type dictates the optimal touch DNA preprocessing method for maximizing DNA yield and quality.
Findings provide a theoretical framework for touch DNA sample handling in casework.
Recommendations offered for selecting appropriate methods in forensic investigations.