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Diagnosis of pseudorabies virus infection in pigs with specific DNA probes

T Linné1

  • 1Department of Veterinary Microbiology, Biomedical Centre, Uppsala, Sweden.

Research in Veterinary Science
|September 1, 1987
PubMed

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...