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Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
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Demineralized bone matrix used for direct pulp capping in rats.
Qian Liu1,2, Yanhong Ma3, Junlan Wang1,2
1Key Laboratory of Oral Diseases of Jiangsu Province and Stomatological School of Nanjing Medical University, Nanjing, Jiangsu, China.
Plos One
|March 3, 2017
Summary
Demineralized bone matrix (DBM) promotes better wound healing and reparative dentin formation compared to calcium hydroxide for direct pulp capping. DBM shows potential as an effective alternative for dental pulp treatment.
Area of Science:
- Biomaterials Science
- Dental Regenerative Medicine
- Tissue Engineering
Background:
- Direct pulp capping is a dental procedure to preserve pulp vitality.
- Calcium hydroxide (Ca(OH)2) is a traditional material, but alternatives are sought.
- Demineralized bone matrix (DBM) is a biomaterial with regenerative potential.
Purpose of the Study:
- To compare the efficacy of demineralized bone matrix (DBM) with calcium hydroxide (Ca(OH)2) in promoting pulp wound healing.
- To evaluate the formation of reparative dentin after direct pulp capping with DBM and Ca(OH)2.
- To assess the expression of key dentinogenesis markers.
Main Methods:
- Pulpotomy was performed on rat molars, followed by capping with either DBM or Ca(OH)2.
- Histological analysis using Hematoxylin-Eosin staining.
- Immunohistochemical analysis for runt transcription factor 2 (Runx2), type I collagen (COL I), osteocalcin (OCN), and dentin sialoprotein (DSP).
Main Results:
- DBM group exhibited reduced inflammatory cell infiltration and pulp tissue disorganization compared to Ca(OH)2.
- Reparative dentin formation was observed earlier and was more organized with DBM.
- DBM group showed stronger expression of Runx2, COL I, OCN, and DSP, indicating enhanced dentinogenesis.
Conclusions:
- DBM effectively induces odontoblast differentiation and promotes dentinogenesis.
- DBM facilitates physiologic wound healing and reparative dentin formation in pulp capping.
- DBM presents a promising alternative material for direct pulp capping procedures.
