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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Mar 6, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

A Lentiviral Fluorescent Genetic Barcoding System for Flow Cytometry-Based Multiplex Tracking.

Tobias Maetzig¹, Jens Ruschmann², Courteney K Lai²

¹Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada; Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany.

Molecular Therapy : the Journal of the American Society of Gene Therapy

|March 3, 2017

Summary

This summary is machine-generated.

Researchers developed novel lentiviral fluorescent genetic barcoding (FGB) systems for real-time cell tracking. These FGB systems enable multiplex clonal fate mapping and reduce sample numbers, advancing cell population analysis.

Keywords:

fluorescent genetic barcoding hematopoietic stem cells lentiviral gene transfer multiplexing

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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

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Published on: July 13, 2019

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Retroviral integration site analysis is crucial for clonal fate mapping but involves delays.
Current multiplex assays for real-time cell tracking and competitive growth analysis are limited.

Purpose of the Study:

To develop and validate novel lentiviral fluorescent genetic barcoding (FGB) systems.
To enable real-time monitoring of multiple cell populations in multiplex assays.

Main Methods:

Development of three generations of lentiviral fluorescent genetic barcoding (FGB) vectors.
Flow cytometry for tracking color-coded cell populations in vitro for up to 28 days.
In vitro and in vivo experiments to assess FGB vector functionality.

Main Results:

Successfully created FGB systems with 26, 14, or 6 unique labels.
Demonstrated multiplex tracking of color-coded populations using flow cytometry.
Facilitated in vitro screening of microRNA-induced growth advantages and in vivo recovery of hematopoietic stem cells.

Conclusions:

The novel FGB vectors offer a powerful tool for in vitro and in vivo multiplexing experiments.
These systems enable efficient assessment of comparative cell growth properties.
FGB technology allows for a significant reduction in sample numbers for multiplex analyses.