Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 6, 2026

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.6K

Methods for Studying Interactions Between Atg8/LC3/GABARAP and LIR-Containing Proteins.

T Johansen1, Å B Birgisdottir1, J Huber2

  • 1Molecular Cancer Research Group, Institute of Medical Biology, University of Tromsø-The Arctic University of Norway, Tromsø, Norway.

Methods in Enzymology
|March 4, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction notice to "Pharmacokinetics of Implanon: An integrated analysis" [Contraception 58 (1998) S85-S90].

Contraception·2025
Same author

Specific molecular peak analysis by ion mobility spectrometry of volatile organic compounds in urine of COVID-19 patients: A novel diagnostic approach.

Journal of virological methods·2024
Same author

[Report on the 29th annual meeting of the kidney transplantation working group of the German Society of Urology in Marburg 2023].

Urologie (Heidelberg, Germany)·2024
Same author

Musculoskeletal tissue engineering: Adipose derived stromal cell implementation for the treatment of osteoarthritis.

Biomaterials·2022
Same author

Treatment trends for muscle-invasive bladder cancer in Germany from 2006 to 2019.

World journal of urology·2022
Same author

A primer of an in-depth resilience status for German medical graduates: results of a cross-sectional survey on the status quo of resilience among graduates of human medicine in Bavaria, Germany - a necessary step in building an emotionally equipped healthcare work-force.

BMC medical education·2021
Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026
Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026
Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026
Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026
Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026
Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026
See all related articles

LC3/GABARAP proteins mediate selective autophagy by binding cargo receptors via LC3-interacting regions (LIRs). This study presents methods to identify LIR proteins and study their interactions with LC3/GABARAP machinery.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • LC3/GABARAP proteins are essential for autophagosome formation and selective cargo degradation in autophagy.
  • Selective autophagy relies on receptor proteins that link cargo to the LC3/GABARAP machinery.
  • LC3-interacting regions (LIRs) are key motifs mediating these receptor-LC3/GABARAP interactions.

Purpose of the Study:

  • To provide robust protocols for identifying LIR-containing proteins.
  • To detail methods for characterizing the interactions between LIR motifs and LC3/GABARAP proteins.
  • To advance the study of selective autophagy mechanisms.

Main Methods:

  • Yeast-two-hybrid screening
  • Glutathione S-transferase (GST) pulldown assays
Keywords:
Atg8GABARAPGST pulldownITCLC3LIRPeptide arrayUBLYeast-two-hybrid

More Related Videos

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells
09:51

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

1.5K
Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
07:20

Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

2.8K

Related Experiment Videos

Last Updated: Mar 6, 2026

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.6K
Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells
09:51

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

1.5K
Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
07:20

Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

2.8K
  • Peptide arrays (including 2D arrays)
  • Biophysical interaction studies
  • LIR peptide preparation and purification

    • Main Results:

    • Established protocols for identifying LIR-containing proteins using multiple biochemical and screening methods.
    • Demonstrated the utility of 2D peptide arrays for pinpointing critical LIR residues.
    • Described a biophysical approach to quantify LC3/GABARAP and LIR protein interactions.

    Conclusions:

    • The presented methods facilitate the discovery and characterization of novel selective autophagy receptors.
    • Understanding LIR-protein interactions is crucial for dissecting the specificity of the autophagy pathway.
    • These protocols offer valuable tools for researchers investigating autophagy and related cellular processes.