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Oligopeptide Competition Assay for Phosphorylation Site Determination
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Allosteric Modulation of AMPK Enzymatic Activity: In Vitro Characterization

J Ward1, A R Reyes1, R G Kurumbail2

  • 1Cardiovascular and Metabolic Diseases Research Unit, Pfizer Worldwide Research and Development, Cambridge, MA, United States.

Methods in Enzymology
|March 4, 2017
PubMed
Summary

AMP-activated protein kinase (AMPK) is a crucial cellular energy sensor. This study details assay methods for discovering and characterizing small-molecule AMPK activators, vital for understanding cellular metabolism and stress responses.

Keywords:
AMPK activatorAMPK in autophagyAMPK in vitro assaysEnzyme activation

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