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Synthesis in Escherichia coli of the HTLV-I trans-acting protein p40x
A Sánchez1, M A Richardson, M Yoshida
1Department of Molecular Genetics, Nippon Roche Research Center, Kanagawa, Japan.
Virology
|December 1, 1987
Abstract:
The pX gene of human T-cell leukemia virus type I (HTLV-I) encodes a protein that activates the expression of viral genes in trans. Plasmid constructs designed to express the pX gene under the control of either the temperature-inducible lambda PL promoter or the trp promoter were used to transform several Escherichia coli strains, including murein-lipoprotein and Ion mutant strains. Upon induction it was possible to detect the synthesis of a new polypeptide of approximately 40 kDa which reacted specifically with serum from an ATL patient.