Related Experiment Video
Updated: Mar 6, 2026
06:44
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Published on: April 28, 2021
4.7K
Reverse the Resistance to PARP Inhibitors
Yevgeniy Kim1, Aleksei Kim1, Ainur Sharip1
1Department of Biology, Nazarbayev University, School of Science and Technology, Astana, 010000, Republic of Kazakhstan.
International Journal of Biological Sciences
|March 4, 2017
Summary
Poly (ADP-ribose) Polymerase (PARP) inhibitors are vital cancer treatments. This review details recent molecular mechanisms of PARP inhibitor resistance and strategies to overcome it.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- Poly (ADP-ribose) Polymerase (PARP) enzymes are crucial for DNA repair, particularly single-strand breaks.
- PARP inhibitors (PARPi) are established as a valuable addition to conventional chemotherapy for cancer treatment.
- Acquired resistance to PARPi poses a significant challenge in clinical oncology.
Purpose of the Study:
- To elucidate the latest molecular mechanisms underlying resistance to PARP inhibitors.
- To summarize emerging strategies for reversing PARP inhibitor resistance in cancer therapy.
Main Methods:
- Literature review of recent studies on PARPi resistance.
- Analysis of molecular alterations and genetic events conferring resistance.
- Synthesis of data on therapeutic approaches to overcome resistance.
Main Results:
- Identified several novel molecular mechanisms driving PARPi resistance, including specific gene mutations and pathway alterations.
- Documented various strategies, such as combination therapies and targeting resistance pathways, to restore PARPi efficacy.
- Highlighted the dynamic and complex nature of resistance development.
Conclusions:
- Understanding the molecular basis of PARPi resistance is critical for developing effective treatment strategies.
- Targeting resistance mechanisms offers promising avenues to improve patient outcomes in PARPi-treated cancers.
- Further research is needed to translate these findings into clinical practice.
More Related Videos
08:46Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
Published on: December 9, 2015
11.2K
10:44Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Published on: January 31, 2018
10.8K
Related Concept Videos
02:56Treatment Resistant Cancers
3.8K
Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.8K
02:37Restarting Stalled Replication Forks
6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
02:57Targeted Cancer Therapies
9.0K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
9.0K
02:36DNA Damage can Stall the Cell Cycle
10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
02:36DNA Damage Can Stall the Cell Cycle
3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
01:02Long-patch Base Excision Repair
8.2K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
8.2K