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Visualization of ANP receptor on glomeruli of bovine kidney by use of a specific antiserum
M Shimonaka1, T Saheki, H Hagiwara
1Department of Chemistry, Tokyo Institute of Technology, Japan.
The American Journal of Physiology
|November 1, 1987
Abstract:
Atrial natriuretic peptide (ANP) receptor was purified from the bovine lung by affinity chromatography and preparative gel electrophoresis. A specific antiserum was raised against the purified receptor and used to localize receptors immunohistochemically on bovine kidney sections. Intense staining was seen in glomeruli. This distribution corresponds to that of ANP binding sites revealed by previous autoradiographic analyses and matches a glomerular action of ANP suggested by physiological studies.