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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
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Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Generating Conformation and Complex-Specific Synthetic Antibodies.

Marcin Paduch1, Anthony A Kossiakoff2,3

  • 1Department of Biochemistry and Molecular Biology, The University of Chicago, KCBD Rm.3240G, 900 East 57th Street, Chicago, IL, 60637, USA. mpaduch@recombinant-antibodies.org.

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|March 4, 2017
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Summary

This study presents new phage display protocols for creating synthetic antibodies that can target complex protein structures and specific cellular states. These methods aim to isolate effective therapeutic antibodies for various applications.

Keywords:
Antibody phage displayConformation specificSynthetic antibody

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Phage display is a standard technique for identifying binding molecules from large libraries.
  • Developing antibodies for complex biological targets like protein complexes remains a challenge.

Purpose of the Study:

  • To present novel phage display protocols for generating synthetic antibodies.
  • To enable the recognition of multiprotein complexes and conformational states.
  • To provide a framework for isolating bioactive antibodies for therapeutic development.

Main Methods:

  • Detailed protocols for experiment design, optimization, and screening using phage display.
  • Application of methods for direct cell screening.
  • Adaptability of protocols for other in vitro directed evolution systems with non-immunoglobulin scaffolds.

Main Results:

  • Successful generation of synthetic antibodies capable of recognizing multiprotein complexes.
  • Demonstration of protocols for isolating antibodies targeting specific conformational states.
  • Establishment of a framework for downstream assay development.

Conclusions:

  • The presented phage display protocols are effective for generating targeted synthetic antibodies.
  • These methods facilitate the discovery of novel therapeutic antibodies.
  • The protocols are versatile and applicable to cell-based screening and other directed evolution systems.